The Illumina BeadChips described below are available for service at GPCF - Information as provided by Illumina Inc.
The Sentrix BeadChip technology is set up to perform multiple hybridizations in parallel. The Sentrix-6 chip type is most economic with 6 samples (or multiple thereof), and the Sentrix-8 chip type is best used with 8 samples (or multiple thereof) and the 12-chip type should be used with 12 samples (or multiple thereof).
HumanHT-12 v4 Expression BeadChip Kits
The BeadChip HumanHT-12 v4 offers comprehensive analysis of genome-wide expression on a single array.
- probes are defined gene-specific 50mer oligonucleotides
- ca. 300,000 accessible oligonucleotides per bead/spot
- on average 15x redundancy for each transcript
- Provides comprehensive coverage of the transcribed human genome on a single array
- Analyzes the expression level of 31,000 annotated genes with more than 47000 probes derived from the NCBI RefSeq Release 38 (November 7, 2009) and other sources.
- comprised of more than 7,000,000 beads per array, meaning 12x7,000,000 beads per chip
- Up-to-date gene list and annotation of HumanHT-12 v4
For more information please download the HumanHT-12 v4 Whole Genome BeadChip datasheet from Illumina. One physical array is made up of 12 identical, but independent chips.
mouseWG-6 v2 BeadChip®
The BeadChip Mouse Sentrix-6 V2 offers comprehensive analysis of genome-wide expression on a single array.
- probes are defined gene-specific 50mer oligonucleotides
- >700,000 oligonucleotides per bead/spot
- on average 30x redundancy for each transcript
- Provides comprehensive coverage of the transcribed mouse genome on a single array
- Analyzes the expression level of 45,281 mouse transcripts, variants, and EST clusters
- comprised of more than 1,600,000 beads per chip
- Up-to-date gene list and annotation of Mouse Sentrix-6 V2 BeadChip
For more information please download the Mouse Sentrix-6 V2 Whole Genome BeadChip datasheet and the corresponding technical bulletin from Illumina. One physical array is made up of 6 identical, but independent chips.
mouseRef-8 v2 BeadChip®
The BeadChip Mouse RefSeq Sentrix-8 V2 offers comprehensive analysis of genome-wide expression on a single array.
- probes are defined gene-specific 50mer oligonucleotides
- >700,000 oligonucleotides per bead/spot
- on average 30x redundancy for each transcript
- Provides comprehensive coverage of the transcribed mouse genome on a single array
- Analyzes the expression level of 25,697 mouse transcripts and variants, as defined by RefSeqs (NCBI) sequences
- comprised of more than 800,000 beads per chip
- Up-to-date gene list and annotation of Mouse Sentrix-8 RefSeq V2 BeadChip
For more information please download the Mouse Sentrix-8 RefSeq V1.1 BeadChip datasheet and the corresponding technical bulletin from Illumina. One physical array is made up of 8 identical, but independent chips.
RatRef12 BeadChip®
The BeadChip Rat Sentrix-12 V1 offers comprehensive analysis of genome-wide expression on a single array.
- probes are defined gene-specific 50mer oligonucleotides
- >700,000 oligonucleotides per bead/spot
- on average 30x redundancy for each transcript
- Provides comprehensive coverage of the transcribed rat genome on a single array
- Analyzes the expression level of 22,523 rat transcripts and variants, primarily selected from RefSeq (release 16)
- comprised of more than 1,600,000 beads per chip
For more information please download the Rat Sentrix-12 V1 RefSeq BeadChip annotation file, and see the description of the corresponding datasheet.
One physical array is made up of 12 identical whole transcriptome bead arrays that need to be processed in parallel.
