PhD Program: 0,5 Credit Points
The Transgenic Service organizes a yearly course "Generation of transgenic (genetically altered) mice".
This Course is for interested colleagues at the DKFZ.
Registration: At HCM portal (Training and In-service training M300)
Next courses:
Course in German
Part I – Theory January 21st, 2010 9:00 – 12:00 hrs
Part II – Practice January 28th, 2010 8:30 – 13:00 hrs
Course in English
Part I – Theory January 26th, 2010 9:00 – 12:00 hrs
Part II – Practice February 04th, 2010 8:30 – 13:00 hrs
Place
Theory: 8th floor, room-No. 824
Practice: Transgenic Service at the DKFZ, basement, ATV building
Part I – Theory
· Transgenic animal models - for what reason?
· General strategy for generation of transgenic mice
· DNA microinjection into pronucleus of zygotes
· Embryonic stem cells and homologous recombination
· Injection of ES cells into blastocysts
· Comparison of DNA microinjection versus ES cell injection
· Vasectomy of male mice
· Cryopresservation of mouse embryos and sperm
. Cre/loxP and Flp/FRT system
· Alternative methods for generation of transgenic mice
Part II – Practice
· Introduction into animal (mice) housing
· Collection of mouse embryos (zygotes and blastocysts)
· Injection of DNA into pronucleus of zygotes
· Injection of embryonic stem cells into blastocysts
· Transfer of embryos into the oviduct and uterus
· Vasectomy of male mice
Participation of part I is necessary for visiting the practical part.