Liver Controls Cachexia in Cancer
Activated gene in the liver brings blood fat levels down
Many cancer patients suffer from a dramatic loss of fat and muscle tissue. This extreme wasting, or cachexia, is often the actual cause of death in cancer patients. Heidelberg scientists have now discovered in mice that tumors stimulate the production of a key gene switch in the liver. Activity of this switch lowers blood fat levels so that the animals lose weight. The researchers have been the first to demonstrate that the liver controls cancer-induced cachexia. This finding may lead to approaches to slow down this fatal loss of body mass.
Cachexia or wasting affects up to 70 percent of all cancer patients depending on the type of cancer. It is characterized by a dramatic loss of body weight independent of food intake. Cachexia is seen particularly often and most pronounced in patients suffering from cancers of the digestive tract and the lungs. They may lose up to 80 percent of body fat and skeletal muscle. Muscle loss leads to weakness and immobility of patients and poorer response to treatment. An estimated 20 percent of cancer deaths are considered to be a direct consequence of cachexia, with failure of the respiratory muscles as a frequent cause of death.
“Doctors used to believe that cancer re-programs metabolism focusing all energy into tumor growth,” says Prof. Dr. Stephan Herzig, who heads a joint research department of the German Cancer Research Center (DKFZ), Heidelberg University and Heidelberg University Hospital. However, by now researchers presume that cachexia is the body’s response to various harmful stimuli originating directly from the growing tumor. To explore the causes of cachexia, Stephan Herzig, an expert in metabolism, took a closer look at the liver as the key controlling point of metabolism. “Cachexia patients frequently show an inflamed fatty liver – this was a major clue for this organ being involved.”
The researchers found extremely low lipid (blood fat) levels in cancerous mice, therefore they lack the most important energy source. However, they accumulate fat in the liver. The low lipid levels in the diseased animals are due to their liver releasing only very little VLDL (very low density lipoprotein). VLDL facilitates the transport of fat in the bloodstream. Moreover, the genes for all major steps of lipogenesis are blocked in the livers of cancerous mice.
“This is a clear indication of a central gene switch in the liver driving cachexia”, says Stephan Herzig. He therefore searched for differences in protein switches regulating gene activity and hence hepatic energy metabolism in cancerous and healthy mice. Herzig’s team found significant differences in a poorly studied gene switch called TSC22D4, which is found in larger amounts in cancerous mice than in healthy control mice.
Herzig’s team was able to clearly demonstrate the key role of TSC22D4 in the onset of cachexia. When the researchers silenced the switch specifically in the animals’ livers the organ subsequently went back to producing enough VLDL to make lipid levels in the cancerous animals rise. In addition, the genes involved in lipogenesis got boosted again.
“Our results prove, for the first time, that dramatic loss of body mass may be controlled by the liver,” says Stephan Herzig. “We also learned recently that TSC22D4 shows exactly the same effect in human hepatic cells. There is evidence that certain metabolites control this gene switch and might offer the possibility to slow down the fatal wasting process. However, we don´t have proven this approach by now. This is what we will do next.”
Allan Jones, Kilian Friedrich, Maria Rohm, Michaela Schäfer, Carolyn Algire, Philipp Kulozik, Oksana Seibert, Karin Müller-Decker, Tjeerd Sijmonsma, Daniela Strzoda, Carsten Sticht, Norbert Gretz, Geesje M. Dallinga-Thie, Barbara Leuchs, Manfred Kögl, Wolfgang Stremmel, Mauricio Berriel Diaz and Stephan Herzig: Transforming growth factor-beta1 Stimulated Clone-22 D4 is a molecular output of hepatic wasting metabolism, EMBO Molecular Medicine 2012
The German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) with its more than 3,000 employees is the largest biomedical research institute in Germany. At DKFZ, more than 1,000 scientists investigate how cancer develops, identify cancer risk factors and endeavor to find new strategies to prevent people from getting cancer. They develop novel approaches to make tumor diagnosis more precise and treatment of cancer patients more successful. The staff of the Cancer Information Service (KID) offers information about the widespread disease of cancer for patients, their families, and the general public. Jointly with Heidelberg University Hospital, DKFZ has established the National Center for Tumor Diseases (NCT) Heidelberg, where promising approaches from cancer research are translated into the clinic. In the German Consortium for Translational Cancer Research (DKTK), one of six German Centers for Health Research, DKFZ maintains translational centers at seven university partnering sites. Combining excellent university hospitals with high-profile research at a Helmholtz Center is an important contribution to improving the chances of cancer patients. DKFZ is a member of the Helmholtz Association of National Research Centers, with ninety percent of its funding coming from the German Federal Ministry of Education and Research and the remaining ten percent from the State of Baden-Württemberg.