Stimulated Emission Depletion (STED)

© dkfz.de

Once molecular switching is introduced into the imaging process, diffraction of light does no longer limit the resolution of a light microscope.
In STED-microscopy, the fluorescence of molecules outside the very centre of the diffraction limited spot is switched off by a ring-shaped stimulation beam. This enables fluorescence imaging at a resolution of just a few 10nm in biologically intact samples.

Kooperationspartner

Cooperation with University of Heidelberg,Physikalisch-Chemisches Institut - Prof. Dr. J.P. Spatz


Paxilin staining of focal adhesions in primary human fibroblast cells.

STED reveals the Paxilin distribution within the focal contacts.

Cooperation with DKFZ, Department: Molecular Genetics - Prof. Dr. Peter Lichter:

The distribution of sub-units isn't observable

The subunits of Speckles (Splicing Factor SC35) become visible with STED.

Cooperation with Univeristy of Heidelberg, centre for molecular biology (ZMBH) - Dr. S. Kins:

The distribution of APP (Amyloid Precursor Protein) in Neurons is resolved with STED

APP-distribution confocal

2 color STED overlay (APP,Synaptophysin)

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