4Pi Mikroskopie

© dkfz.de

The impact of numerical aperture on the resolution of an optical microscope has been discovered by Abbe in 1876.
In the 4pi optical setup, two opposing micro-objectives increase the aperture to almost the complete solid angle of 4 pi. As a result, axial resolution is improved by a factor of 3 to 5. This enables fluorescence imaging at the 100nm level in 3d.

Cooperations

Cooperation with Max-Planck-Institut for bio-physical Chemistry, Department NanoBiophotonik - Prof. Dr. S.W. Hell:


cis-trans Golgi;Top: confocal, Bottom: 4Pi

Microtubuli in a neuron cell

3d-structure of Microtubuli in the neighborhod of the cell nucleus

3d-structure of Mitochondria in the neighborhod of the cell nucleus

Cooperation with DKFZ, Department Genetics of Skin Carcinogenesis - Prof. Dr. P. Boukamp:

Telomer distribution in the nucleus at 100nm resolution

In cooperation with the DKFZ department Genome Organization and Function - Dr. Karsten Rippe:

Distribution of diverse proteins in the shell of the PML-body

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