A major challenge in cancer cell biology is to understand how cells and molecular processes function and play together as systems. Therefore our studies focus on the interplay between different cellular components to examine how they create modules and systems, and how changes in these modules can lead to cancer, metastasis and drug resistance. In this line, the group members aim to find novel strategies of integrating experimental data and computational biology.

Current projects

• Drug resistance in breast cancer
• Tumor cell/microenvironment interactions
• Cell-cell adhesion in cancer progression

Team

Interactions between glycosaminoglycan degrading enzymes and signaling pathways are studied in a 3D cell culture system | © dkfz.de

Group head:
Dorit Arlt
Post Doc:
Azeemudeen Hussain
PhD student:
Steve Wagner
Diploma student:
Sophie Hetterich

Technicians:
Sandra Blaszkiewicz, Christina Bootz

Former members:
Mamatha Sauermann, Meher Majety, Özgür Sahin

Publications

Hahne F, Mehrle A, Arlt D, Poustka A, Wiemann S, Beissbarth T. Extending pathways based on gene list signatures using InterPro domains. BMC Bioinformatics 2008; 4;9(1):3

Lobke C, Laible M, Rappl C, Ruschhaupt M, Sahin O, Arlt D, Wiemann S, Poustka A, Sultmann H, Korf U. Contact spotting of protein microarrays coupled with spike-in of normalizer protein permits time-resolved analysis of ERBB receptor signaling. Proteomics 2008; 8:1586-94.

Sauermann M, Sahin Ö, Sültmann H, Hahne F, Blaszkiewicz S, Majety M, Füzesi L, Poustka A, Wiemann S, Arlt D. Reduced expression of the Vacuole membrane protein 1 (Vmp1) affects the invasion capacity of tumor cells. Oncogene 2008; 27:1320-6.

Sahin Ö, Löbke C, Korf U, Sültmann H, Poustka A, Wiemann S, Arlt D. Combinatorial RNAi for quantitative protein network analysis. Proc Natl. Acad. Sci. 2007; 104: 6579-84

Sauermann M, Hahne F, Schmidt C, Majety M, Rosenfelder H, Bechtel S, Huber W, Poustka A, Arlt D, Stefan Wiemann. High-throughput flow cytometry-based assay to identify apoptosis-inducing proteins. J Biomol Screen 2007; 12: 510-520.

Arlt D, Sahin O, Korf U, Loebke C, Beissbarth T, Hahne F, Wiemann S, Poustka A. Modeling breast cell cycle regulation - overcoming drug resistance. Conf Proc IEEE Eng Med Biol Soc 2006; 1:40-3.

Hahne F, Arlt D, Sauermann M, Majety M, Poustka A, Wiemann S, Huber W. Statistical methods and software for the analysis of high throughput reverse genetic assays using flow cytometry readouts. Genome Biol. 2006; 7: R77

Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S. The LIFEdb database in 2006. Nucleic Acids Res. 2006; 34: D415-8.

Arlt D, Huber W, Liebel U, Schmidt C, Majety M, Sauermann M, Rosenfelder H, Bechtel S, Mehrle A, Bannasch D, Schupp I, Seiler M, Simpson JC, Hahne F, Moosmayer P, Ruschhaupt M, Guilleaume B, Wellenreuther R, Pepperkok R, Sültmann H, Poustka A, Wiemann S. Functional profiling discovers 16 cancer relevant cell cycle modulators. Cancer Res. 2005; 65: 7733-42.

Guilleaume B, Buness A, Schmidt C, Klimek F, Moldenhauer G, Huber W, Arlt D, Korf U, Wiemann S, Poustka A. Systematic comparison of surface coatings for protein microarrays. Proteomics 2005; 5: 4705-12.

Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sultmann H, Poustka A. From ORFeome to biology: a functional genomics pipeline. Genome Res. 2004; 14: 2136-44.