Imaging and Cytometry

Imaging and Cytometry Core Facility

Prof. Dr. Jürgen Kartenbeck (in ch.)

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Biomedical research benefits greatly from imaging and cell enrichment processes, but scientific results in these fields are reliant upon the efficient and correct use of complex and expensive instrumentation. Dynamic progress in the development of such technology results in a constant requirement for new or updated equipment, as well as experienced staff members able to guarantee the correct, appropriate use of the instruments; execution of the experiments; and data analysis - all of which is time consuming.

In order to minimize such investment of capital and personnel for individual groups, the DKFZ has established the Imaging and Cytometry Core Facility; made up of individual units it provides a wide range of high quality services in four different fields.

Each unit is directed by a highly experienced scientist. The units are responsible not only for the allocation and maintenance of instruments, lab space and to some extent, consumables, but their scientific and technical staff offers training and assistance in sample preparation, instrument application, experimental set-up design, interpretation of the results and detailed, unit-specific seminars.

  1. The Small Animal Imaging unit supports clients with a variety of radiological methods among them, ultrasound, mPET, mCT, mSPECT and MRT instruments to collect and quantify morphological and functional data in vivo.
  2. The FlowCytometry unit performs cell cloning or enrichment of up to 4 subpopulations simultaneously by using several flow cytometric cell sorters (up to 5 laser, cuvette and jet-in-air sorter) enabling analysis of up to 19 parameters per cell.
  3. The Light Microscopy unit provides a number of manual and fully automated wide-field and confocal laser-scanning microscopes. The range of services is supplemented by support for sample preparation (e.g. paraffin and cryo-sectioning, laser microdissection), and spatial visualization of whole-mount specimens, as well as data analysis and digital image processing.
  4. The Electron Microscopy unit offers full-service and guided access in EM preparation and imaging, including conventional Epon-EM, low-temperature EM (high-pressure freezing, freeze substitution and cryo-sectioning), immuno-EM and single particle EM (negative staining, rotary shadowing).

Units:

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