Serology

Infectious agents in highly endemic cancers in Shaanxi province, China.

Shumei Wang




Introduction
Helicobacter pylori infection is strongly associated with severe gastric diseases such as gastric carcinoma. This infection leads to strong antibody response to many bacterial proteins, which could be exploited as diagnostic marker for infection and potentially also for H.pylori-associated diseases. Recent serologic study in our laboratory with VacA of strain 26695 and G27 indicated the presence of strain specific antibody responses, raising the possibility of VacA serotypes.

Objective
The main aim of this project is to identify the region of VacA responsible for strain specific antibody responses, to develop a sensitive and specific serological assay identifying VacA serotypes, and to describe the geographical distribution of VacA serotypes.

Development of multiplex serology for the detection of viral infections in laboratory rodents

Eva Klaessen



Introduction

For animal experiments high standardisation is essential to gain statistically significant data with as less animals as possible. Used animals shall be free of all pathogenic agents to avoid interference with research. Health monitoring according to the FELASA Recommendations is crucial to detect infections in new animals from other institutions and in the housed population as early as possible.

Objective
To make the laborious and expensive serology surveillance of viral infections more efficient, a multiplex assay for all 26 relevant viral pathogens in rodents is being developed.


References

Nicklas, W., P. Baneux, et al. (2002). "Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units." Lab Anim 36(1): 20-42.

Development of serologic assays based on multiplex technology for HIV-1, HTLV-1, HBV, MCV, HPyV6 and HPyV7

Lena Kranz



Introduction

So far, six human viruses have been classified as carcinogenic to humans by the International Agency for Research on Cancer (IARC). About 17 % of the global cancer burden are estimated to be attributable to tumorigenic viruses; it is assumed, however, that this postulated fraction highly underestimates the actual number of virus-associated cancer cases. In about 50 % of all cancer patients, the etiology is not clearly defined as known factors usually underlying tumor development are missing. A fraction of cancers exhibiting increased incident rates among immunosuppressed individuals are potentially associated with viral infections. Despite ubiquitous appearance of most human cancer viruses, tumor development is a very rare event in infected individuals, and the onset of cancer typically occurs not until decades after primary infection with a transforming virus.

Objectives

In this study, the repertoire of cancer-associated pathogens already established for multiplex technology is increased through the development of serologic assays allowing the simultaneous detection of antibodies arising in infections with human immune deficiency virus 1 (HIV-1), human T lymphotropic virus 1 (HTLV-1) hepatitis B virus (HBV), Merkel cell polyomavirus (MCV), and human polyomavirus 6 and 7 (HPyV6 and HPyV7). Upon technical assay validation, potential causal relationships will be analysed in large epidemiological studies.

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